منابع مشابه
Conformational Changes in Ribosomal Ribonucleic Acid
Both rat liver and Escherichia coli rRNA in 0.1M-sodium chloride were titrated with acid or alkali over the range pH 3-7 at approx. 0°C. rRNA did not bind acid reversibly and hysteresis was observed, i.e. the plot of acid bound to rRNA against pH had the form of a loop showing that the amount of acid bound at a particular pH depended on the direction of the titration. Although the boundary curv...
متن کاملMolecular integrity of plant ribosomal ribonucleic Acid.
Thermal denaturation of plant ribosomal RNA followed by gel fractionation shows that although a large percentage of molecules contain breaks in the polynucleotide chain, 25S and 18S RNAs do exist as unique molecular species. Values for the rate constant of hydrolysis under routine denaturing conditions are of the order of 10(-7) to 10(-8) sec(- 1) and these are shown not to be a result of ribon...
متن کاملRibonucleic acid metabolism in lymphocytes stimulated by phytohemagglutinin. II. Rapidly synthesized ribonucleic acid and te production of ribosomal ribonucleic acid.
Human peripheral lymphocytes are stimulated to change, in vitro, from a resting state to one of progressive enlargement, culminating in mitosis, by treatment with Phaseolus vulgaris phytohemagglutinin. The sedimentation characteristics and fate of rapidly synthesized RNA have been studied in this system after 20 hours of stimulation with phytohemagglutinin, a time when RNA synthesis has been ac...
متن کاملRelationship between sporulation-specific 20S ribonucleic acid and ribosomal ribonucleic acid processing in Saccharomyces cerevisiae.
The nature and properties of the 20S ribonucleic acid which accumulates only during the sporulation of Saccharomyces cerevisiae were examined. The 20S ribonucleic acid (RNA) has a base composition considerably different from ribosomal RNA species and is virtually unmethylated. The 20S RNA did, however, exhibit approximately 70% homology with 18S RNA by RNA-deoxyribonucleic acid filter hybridiza...
متن کاملStructural analyses of mammalian ribosomal ribonucleic acid and its precursors. Nucleotide sequence of ribosomal 5.8 S ribonucleic acid.
The nucleotide sequence of ribosomal 5.8 S RNA (also known as 7 S or 5.5 S rRNA) from Novikoff hepatoma ascites cells has been determined to be (see article). Estimations of the secondary structure based upon maximized base pairing and the fragments of partial ribonuclease digestion indicate that there may be five base-paired regions in the molecule, three forming a folding of the termini and t...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1972
ISSN: 0306-3283
DOI: 10.1042/bj1290035pa